If you want to run miRNAFold with multifasta file, please click on the following link: NEW: Multifasta file
A list of features used for the verification is available here : List of features used
The artificial sequence was created by the concatenation of human mRNAs and the insertion of 100 human pre-miRNAs.
The mRNA sequences came from the Human genome (build 37.2) of the NCBI Website and the pre-miRNAs came from miRBase database (release 17).
Pre-miRNA lengths are from 63 to 110 nucleotides and the start position of pre-miRNAs begins every 300 nucleotides, the first pre-miRNA starting at position 300.
The total length of the artificial sequence is 30 500 nucleotides.