EvryRNA : miRNAFold

Ab initio miRNA prediction in genomes

Multifasta file

If you want to run miRNAFold with a single sequence, please click on the following link: Single sequence

MULTIFASTA file (100 MB max):

Use example file:

Sliding window size: Select the size of the searching window on the genome (default value:150)

Minimum hairpin size: Select the minimal size of the hairpins that miRNAFold will search (default value: 0)

Maximal thermodynamic value of hairpins: Select the maximal energy of hairpins that miRNAFold will search (default value:0)

Percentage of verified features: Select the percentage of verified features, this parameter will change the number of results. (default value:70)

Species Parameters: Select the species parameters which correspond to where your sequence comes from (default value: All miRBase)

A list of features used for the verification is available here : List of features used

Example file

Multiple artificial sequence
- The artificial sequence was created by the concatenation of human mRNAs and the insertion of 100 human pre-miRNAs.
- The mRNA sequences came from the Human genome (build 37.2) of the NCBI Website and the pre-miRNAs came from miRBase database (release 17).
- This multifasta file contains 11 sequences.

For any questions, comments or suggestions about miRNAFold, please feel free to contact: fariza.tahi@ibisc.univ-evry.fr